| 1. |
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Studies on bio-analytical chemistry (Key Word : ) |
| Research Topic | Studies on bio-analytical chemistry | | Research Type | | |
| 2. |
2017/04～2022/03
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Development of micro total analysis system for biological compounds (Key Word : ) |
| Research Period | 2017/04/01～2022/03/31 | | Research Topic | Development of micro total analysis system for biological compounds | | Research Type | KAKENHI Research | | Consignor | Japan Society for the Promotion of Science | | Research Program Type | Grant-in-Aid for Scientific Research (C) | | Research Institute | The University of Tokyo | | Researcher/Collaborator | Tsunoda Makoto | | Details | A technology to enable quantification of trace amounts of biological compounds using pillar array columns as a high-performance on-chip liquid chromatography separation medium was developed. So far, fundamental technologies for separation of biological compounds on pillar array columns have been established. In this study, the sample injection part was investigated as one of the fundamental technologies for quantitative analysis. An automated sample injection system was developed with good reproducibility. Reproducible quantification of amino acids in plasma samples has become possible. | | Permalink URL | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K08234 | |
| 3. |
2014/04～2017/03
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Development of analytical methods for biological compounds suing pillar array columns (Key Word : ) |
| Research Period | 2014/04/01～2017/03/31 | | Research Topic | Development of analytical methods for biological compounds suing pillar array columns | | Research Type | KAKENHI Research | | Consignor | Japan Society for the Promotion of Science | | Research Program Type | Grant-in-Aid for Scientific Research (C) | | Research Institute | The University of Tokyo | | Researcher/Collaborator | Tsunoda Makoto | | Details | Liquid chromatography is widely used for analysis of biological compounds. However, it is known that conventional chromatography has limitation of separation efficiency. Hence, I focused on pillar array column, which is theoretically proven to have higher separation ability. In this study, Low pressure-drop and low-dispersion turn was successfully developed. | | Permalink URL | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26460033 | |
| 4. |
2011～2012
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Development of analytical method for biological compounds using on-chip liquid chromatography (Key Word : ) |
| Research Period | 2011～2012 | | Research Topic | Development of analytical method for biological compounds using on-chip liquid chromatography | | Research Type | KAKENHI Research | | Consignor | Japan Society for the Promotion of Science | | Research Program Type | Grant-in-Aid for Young Scientists (B) | | Research Institute | The University of Tokyo | | Researcher/Collaborator | TSUNODA Makoto | | Details | High-performance liquid chromatography is an important separation technique used for bioanalysis. In this study, we used pillar array column, which has high separation efficiency. We performed fast and quantitative analysis of branched-chain amino acids in biological samples using a pillar array column and Integration of pillar array columns into a gradient elution system for pressure-driven liquid chromatography. | | Permalink URL | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790041 | |
| 5. |
2009～2010
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Development of a sensitive method for catecholamines using on-chip liquid chromatography (Key Word : ) |
| Research Period | 2009～2010 | | Research Topic | Development of a sensitive method for catecholamines using on-chip liquid chromatography | | Research Type | KAKENHI Research | | Consignor | Japan Society for the Promotion of Science | | Research Program Type | Grant-in-Aid for Young Scientists (B) | | Research Institute | The University of Tokyo | | Researcher/Collaborator | TSUNODA Makoto | | Details | The separation efficiency of a pillar array column under pressure-driven liquid chromatography conditions can be improved using a separation channel with low-dispersion turns. The pillar array column was fabricated by reactive ion etching of a silicon substrate. Two coumarin dyes were well resolved under reversed-phase conditions with a maximum theoretical plate number of 8000. Successful separation of the fluorescent derivatives of six amino acids was achieved in 140 s. | | Permalink URL | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790033 | |
| 6. |
2005～2008
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Analyses of functions and interactions of biomolecules by microand nano-devices (Key Word : ) |
| Research Period | 2005～2008 | | Research Topic | Analyses of functions and interactions of biomolecules by microand nano-devices | | Research Type | KAKENHI Research | | Consignor | Japan Society for the Promotion of Science | | Research Program Type | Grant-in-Aid for Scientific Research (A) | | Research Institute | The University of Tokyo | | Researcher/Collaborator | FUNATSU Takashi,SANTA Tomofumi,TSUNODA Makoto,UENO Taro | | Permalink URL | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17201031 | |
| 7. |
2005～2006
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Development of microchip liquid chromatography for ultra-sensitive analysis of trace amounts of bio-molecules. (Key Word : ) |
| Research Period | 2005～2006 | | Research Topic | Development of microchip liquid chromatography for ultra-sensitive analysis of trace amounts of bio-molecules. | | Research Type | KAKENHI Research | | Consignor | Japan Society for the Promotion of Science | | Research Program Type | Grant-in-Aid for Scientific Research (C) | | Research Institute | The University of Tokyo | | Researcher/Collaborator | SANTA Tomofumi,TSUNODA Makoto,FUNATSU Takashi | | Details | The purpose of this study is development of microchip liquid chromatography for ultra-sensitive analysis of trace amounts of bio-molecules. At first, water-soluble fluorescent derivatization regents having benzofurazan structure were developed. These reagents are expected to prevent the adsorption of the analytes to the analytical instruments and improve the sensitivity. Next, fluorescent bi-cyclic nucleic acid, deoxycitidine derivative was synthesized. This structure might be useful as the fluorescent derivatization reagents. Using the synthesized reagents and high-performance liquid chromatography, we have developed the sensitive determination methods of bio-molecules and drugs. These methods are applicable for the microanalysis using microchip liquid chromatography. Mass spectrometry(MS) is promising for the detection method of microchip liquid chromatography. Thus, the derivatization reagents for MS were developed and applied to the analysis of bio-molecules such as fatty acids in biological samples. These methods are also applicable for the microchip liquid chromatography. For the small scale separation, we have synthesized monolithic column in capillary column and on microchip. Finally, we have tried to develop the fluorescence detection system by laser excitation. The compounds near the object lens are sensitively detectable. Therefore, the narrow space less than 5 μm was prepared in the flow area of the microchip and used for the laser excitation fluorescence detection. This method enabled the sensitive detection of the fluorescent compounds. In the future, the methods developed in this study, the derivatization reagents, the micro separation monolithic columns and the fluorescent micro detection system, will be summarized in the microanalysis using microchip liquid chromatography. | | Permalink URL | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17590030 | |
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