ヌノシバ，タツオ   NUNOSHIBA, Tatsuo   布柴　達男    所属   国際基督教大学教養学部 アーツ・サイエンス学科    職種   教授
言語種別	英語
発行・発表の年月	2019/04
形態種別	研究論文（学術雑誌）
査読	査読あり
標題	Development of a supF-based system for detection of mutations in the extreme thermophile Thermus thermophilus HB27.
執筆形態	共著
掲載誌名	Mol Genet Genomics, 294, 1085-1093 (2019)
掲載区分	国外
巻・号・頁	294,pp.1085-1093
著者・共著者	Togawa Y, Shiotani S, Kato Y, Ezaki K, Nunoshiba T, Hiratsu K.
概要	We developed a supF-based system to detect a broad spectrum of mutations in T. thermophilus. This system was validated by measuring spontaneous mutations in the wild type and a udgA, B double mutant deficient in uracil-DNA glycosylase (UDG) activity. We found that the mutation frequency of the udgA, B strain was 4.7-fold higher than that of the wild type and G:C→A:T transitions dominated, which was the most reasonable for the mutator phenotype associated with the loss of UDG function in T. thermophilus. These results show that this system allowed for the rapid analysis of mutations in T. thermophilus, and may be useful for studying the molecular mechanisms responsible for DNA repair and mutagenesis in this extreme thermophile.